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goat anti human il10  (R&D Systems)


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    R&D Systems goat anti human il10
    Goat Anti Human Il10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+il10+antibodies/Human+IL-10+Biotinylated+Antibody/pm30692604-231-39-45
    Average 93 stars, based on 53 article reviews
    goat anti human il10 - by Bioz Stars, 2026-09
    93/100 stars

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    Blocking Assay:

    Article Title: Helper-dependent adenoviral vector achieves prolonged, stable expression of interleukin-10 in rabbit carotid arteries but does not limit early atherogenesis.
    Article Snippet: Conditioned medium samples (50 ml) were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) (0.1% SDS, 15% polyacrylamide), and proteins were transferred to an Immobilon-P membrane (Millipore). .. After blocking membranes with 10 mM Tris-HCl buffer, pH 8.0, containing 150 mM sodium chloride, 0.1% Tween 20, and 5% (w/v) nonfat dry milk, IL-10 protein was detected by incubation with goat anti-human IL10 antibodies (R&D Systems, Minneapolis, MN) followed by peroxidase-conjugated donkey anti-goat secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) and detection of bound antibody by chemiluminescence (ImmunStar WesternC kit; Bio-Rad Laboratories, Hercules, CA). ..

    Incubation:

    Article Title: Helper-dependent adenoviral vector achieves prolonged, stable expression of interleukin-10 in rabbit carotid arteries but does not limit early atherogenesis.
    Article Snippet: Conditioned medium samples (50 ml) were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) (0.1% SDS, 15% polyacrylamide), and proteins were transferred to an Immobilon-P membrane (Millipore). .. After blocking membranes with 10 mM Tris-HCl buffer, pH 8.0, containing 150 mM sodium chloride, 0.1% Tween 20, and 5% (w/v) nonfat dry milk, IL-10 protein was detected by incubation with goat anti-human IL10 antibodies (R&D Systems, Minneapolis, MN) followed by peroxidase-conjugated donkey anti-goat secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) and detection of bound antibody by chemiluminescence (ImmunStar WesternC kit; Bio-Rad Laboratories, Hercules, CA). ..



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    FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
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    FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).

    Journal: Human gene therapy

    Article Title: Helper-dependent adenoviral vector achieves prolonged, stable expression of interleukin-10 in rabbit carotid arteries but does not limit early atherogenesis.

    doi: 10.1089/hum.2010.175

    Figure Lengend Snippet: FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).

    Article Snippet: After blocking membranes with 10 mM Tris-HCl buffer, pH 8.0, containing 150 mM sodium chloride, 0.1% Tween 20, and 5% (w/v) nonfat dry milk, IL-10 protein was detected by incubation with goat anti-human IL10 antibodies (R&D Systems, Minneapolis, MN) followed by peroxidase-conjugated donkey anti-goat secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) and detection of bound antibody by chemiluminescence (ImmunStar WesternC kit; Bio-Rad Laboratories, Hercules, CA).

    Techniques: In Vitro, Expressing, Plasmid Preparation, Cloning, Virus, Transduction, Western Blot, Marker